Vertebrate Cryobiology
Books
Lee, R. E., G. J. Warren and L. V. Gusta (eds.). 1995. Biological Ice Nucleation and its Applications. APS Press, St. Paul. 370 pp.
Invited Reviews & Book Chapters
(student authors in boldface)
Baker, P. J., J. P. Costanzo and R. E. Lee. 2004. Environmental physiology of terrestrial hibernation in hatchling turtles. In: Life in the Cold: Evolution, Mechanisms, Adaptation, and Application (eds. B. M. Barnes and H. V. Carey), pp. 329-338. Twelfth International Hibernation Symposium. Biological Papers of the University of Alaska. Number 27. Institute of Arctic Biology, University of Alaska, Fairbanks, Alaska, USA. | PDF
Lee, R. E. and J. P. Costanzo. 1998. Biological ice nucleation and ice distribution in cold-hardy ectothermic animals. Annual Review of Physiology 60: 55-72. | PDF
Costanzo, J. P. and R. E. Lee. 1996. Mini-review: ice nucleation in freeze-tolerant vertebrates. CryoLetters 17: 111-118. | PDF
Costanzo, J. P. and R. E. Lee. 1995. Supercooling and ice nucleation in vertebrate ectotherms. In: Biological Ice Nucleation and its Applications (eds. R. E. Lee, G. J. Warren and L. V. Gusta), pp. 221-238. APS Press, St. Paul. | PDF
Costanzo, J. P., R. E. Lee, A. L. DeVries, T. Wang and J. R. Layne. 1995. Survival mechanisms of vertebrate ectotherms at subfreezing temperatures: applications in cryomedicine. FASEB Journal 9: 351-358. | PDF
Layne, J. R. and R. E. Lee. 1995. Adaptations of frogs to survive freezing. Climate Research 5: 53-59. | PDF
Costanzo, J. P. and R. E. Lee. 1994. Biophysical and physiological responses promoting freeze tolerance in vertebrates. News in Physiological Sciences 9: 252-256. | PDF
Lee, R. E. and J. P. Costanzo. 1993. Integrated physiological responses promoting anuran freeze tolerance. In: Life in the Cold: Ecological, Physiological, and Molecular Mechanisms (eds. C. Carey, G. L. Florant, B. A. Wunder and B. Horwitz), pp. 501-510. Westview Press, Boulder. | PDF
Lee, R. E. 1988. Freeze tolerance and adaptation to low temperature in insects and frogs. In: Low Temperature Biotechnology: Emerging Applications and Engineering Contributions BED Vol. 10, HTD Vol. 98 (eds. J. J. McGrath and K. R. Diller), pp. 81-90. American Society of Mechanical Engineers, New York. | PDF
Refereed Articles
(student authors in boldface)
Costanzo, J. P., R. E. Lee and G. R. Ultsch. 2008. Physiological ecology of overwintering in hatchling turtles. Journal of Experimental Zoology 309A: 297-379. | PDF
Costanzo, J. P., M. Marjanovic, E.A. Fincel and R. E. Lee. 2008. Urea loading enhances postfreeze performance of frog skeletal muscle. Journal of Comparative Physiology B 178:413-420. | PDF
Muir, T. J., J. P. Costanzo and R. E. Lee. 2008. Metabolic depression induced by urea in organs of the wood frog, Rana sylvatica: effects of season and temperature. Journal of Experimental Zoology 309A:111-116. | PDF
Baker, P. J., J. P. Costanzo and R. E. Lee. 2007. Oxidative stress and antioxidant capacity of a terrestrially hibernating hatchling turtle. Journal of Comparative Physiology B 177:875-883. | PDF
Muir, T. J., J. P. Costanzo and R. E. Lee. 2007. Osmotic and metabolic responses to dehydration and urea-loading in a dormant, terrestrially hibernating frog. Journal of Comparative Physiology B 177:917-926. | PDF
Costanzo, J. P., P. J. Baker and R. E. Lee. 2006. Physiological responses to freezing in hatchlings of freeze-tolerant and -intolerant turtles. Journal of Comparative Physiology B 176: 697-707. | PDF
Baker, P. J., J. P. Costanzo, R. Herlands, R. C. Wood and R. E. Lee. 2006. Inoculative freezing promotes winter survival in hatchling diamondback terrapin, Malaclemys terrapin. Canadian Journal of Zoology 84: 116-124. | PDF
Costanzo, J. P. and R. E. Lee. 2005. Cryoprotection by urea in a terrestrially hibernating frog. The Journal of Experimental Biology 208: 4079-4089. | PDF
Dinkelacker, S. A., J. P. Costanzo, J. B. Iverson and R. E. Lee. 2005. Survival and physiological responses of hatchling Blanding's turtles (Emydoidea blandingii) to submergence in normoxic and hypoxic water under simulated winter conditions. Physiological and Biochemical Zoology 78:356-363. | PDF
Dinkelacker, S. A., J. P. Costanzo and R. E. Lee. 2005. Anoxia tolerance and freeze tolerance in hatchling turtles. Journal of Comparative Physiology B 175:209-217. | PDF
Dinkelacker, S. A., J. P. Costanzo, J. B. Iverson and R. E. Lee. 2004. Cold-hardiness and dehydration resistance of hatchling Blanding’s turtles (Emydoidea blandingii): implications for overwintering in a terrestrial habitat. Canadian Journal of Zoology 82: 594-600. | PDF
Costanzo, J. P., S. A. Dinkelacker, J. B. Iverson and R. E. Lee. 2004. Physiological ecology of overwintering in the hatchling painted turtle: multiple-scale variation in response to environmental stress. Physiological and Biochemical Zoology 77: 74-99. | PDF
Baker, P. J., J. P. Costanzo, J. B. Iverson and R. E. Lee. 2003. Adaptations to terrestrial overwintering of hatchling northern map turtles, Graptemys geographica. Journal of Comparative Physiology B 173: 643-651. | PDF
Costanzo, J. P., P. J. Baker, S. A. Dinkelacker and R. E. Lee. 2003. Endogenous and exogenous ice-nucleating agents constrain supercooling in the hatchling painted turtle. Journal of Experimental Biology 206: 477-485. | PDF
Irwin, J. T., J. P. Costanzo and R. E. Lee. 2003. Postfreeze reduction of locomotor endurance in the freeze-tolerant wood frog, Rana sylvatica. Physiological and Biochemical Zoology 76: 331-338. | PDF
Irwin, J. T. and R. E. Lee. 2003. Geographic variation in energy storage and physiological responses to freezing in the gray treefrogs Hyla versicolor and H. chrysoscelis. Journal of Experimental Biology 206: 2859-2867. | PDF
Costanzo, J. P., E. E. Jones and R. E. Lee. 2001. Physiological responses to supercooling and hypoxia in the hatchling painted turtle, Chrysemys picta. Journal of Comparative Physiology B 171: 335-340. | PDF
Costanzo, J. P., J. D. Litzgus, J. B. Iverson and R. E. Lee. 2001. Cold-hardiness and evaporative water loss in hatchling turtles. Physiological and Biochemical Zoology 74: 510-519. | PDF
Costanzo, J. P., J. D. Litzgus, J. L. Larson, J. B. Iverson and R. E. Lee. 2001. Characteristics of nest soil, but not geographic origin, influence cold hardiness of hatchling painted turtles. Journal of Thermal Biology 26: 65-73. | PDF
Costanzo, J. P., J. D. Litzgus, J. B. Iverson and R. E. Lee. 2000. Seasonal changes in physiology and development of cold hardiness in the hatchling painted turtle, Chrysemys picta. Journal of Experimental Biology 203: 3459-3470. | PDF
Frisbie, M. P., J. P. Costanzo and R. E. Lee. 2000. Physiological and ecological aspects of low-temperature tolerance in embryos of the wood frog, Rana sylvatica. Canadian Journal of Zoology 78: 1032-1041. | PDF
Costanzo, J. P., J. D. Litzgus, J. B. Iverson and R. E. Lee. 2000. Ice nuclei in soil compromise cold hardiness of hatchling painted turtles, Chrysemys picta. Ecology 81: 346-360. | PDF
Woodhams, D. C., J. P. Costanzo, J. D. Kelty
and R. E. Lee. 2000. Cold hardiness in two helminth parasites of
the freeze-tolerant wood frog, Rana sylvatica. Canadian
Journal of Zoology 78: 1085- 1091.
| PDF
Litzgus, J. D., J. P. Costanzo, R. J. Brooks and R. E. Lee. 1999. Phenology and ecology of hibernation in spotted turtles (Clemmys guttata) near the northern limit of their range. Canadian Journal of Zoology 77: 1348-1357. | PDF
Irwin, J. T., J. P. Costanzo and R. E. Lee. 1999. Terrestrial hibernation in the northern cricket frog, Acris crepitans. Canadian Journal of Zoology 77: 1240-1246. | PDF
Costanzo, J. P., J. D. Litzgus and R. E. Lee. 1999. Behavioral responses of hatchling painted turtles (Chrysemys picta) and snapping turtles (Chelydra serpentina) at subzero temperatures. Journal of Thermal Biology 24: 161-166. | PDF
Costanzo, J. P., A. A. Allenspach and R. E. Lee. 1999. Electrophysiological and ultrastructural correlates of cryoinjury in sciatic nerve from the freeze-tolerant wood frog, Rana sylvatica. Journal of Comparative Physiology B 169: 351-359. | PDF
Costanzo, J. P., J. M. Bayuk and R. E. Lee. 1999. Inoculation by environmental ice nuclei in the freeze-tolerant wood frog, Rana sylvatica. Journal of Experimental Zoology 284: 7-14. | PDF
Layne, J. R., J. P. Costanzo and R. E. Lee. 1998. Freeze duration influences postfreeze survival in the frog Rana sylvatica. Journal of Experimental Zoology 290: 197-201. | PDF
Beesley, S. G., J. P. Costanzo and R. E. Lee. 1998. Cryopreservation of spermatozoa from freeze-tolerant and -intolerant anurans. Cryobiology 37: 155-162. | PDF
Costanzo, J. P., J. D. Litzgus, J. B. Iverson and R. E. Lee. 1998. Soil hydric characteristics and environmental ice nuclei influence supercooling capacity of hatchling painted turtles, Chrysemys picta. Journal of Experimental Biology 201: 3105-3112. | PDF
Costanzo, J. P., J. A. Mugnano, H. M. Wehrheim and R. E. Lee. 1998. Osmotic and freezing tolerance in spermatozoa of freeze-tolerant and -intolerant frogs. American Journal of Physiology 275: R713-R719. | PDF
Mugnano, J. A., J. P. Costanzo, S. G. Beesley and R. E. Lee. 1998. Evaluation of glycerol and dimethyl sulfoxide for the cryopreservation of spermatozoa from the wood frog (Rana sylvatica). CryoLetters 19: 249-254. | PDF
Costanzo, J. P., J. T. Irwin and R. E. Lee. 1997. Freezing impairment of male reproductive behaviors of the freeze-tolerant wood frog, Rana sylvatica. Physiological Zoology 70: 158-16. | PDF
Costanzo, J. P., P. A. Callahan, R. E. Lee and M. F. Wright. 1997. Frogs reabsorb glucose from urinary bladder. Nature 389: 343-344. | PDF
Layne, J. R., R. E. Lee and M. Cutwa. 1996. Post-hibernation excretion of glucose in urine of the freeze tolerant frog Rana sylvatica. Journal of Herpetology 30: 85-87. | PDF
Costanzo, J. P. and R. E. Lee. 1996. Mini-review: Ice nucleation in freeze-tolerant vertebrates. CryoLetters 17: 111-118. | PDF
Costanzo, J. P., R. E. Lee, A. L. DeVries, T. Wang and J. R. Layne. 1995. Survival mechanisms of vertebrate ectotherms at subfreezing temperatures: Applications in cryomedicine. FASEB Journal 9: 351-358. | PDF
Mugnano, J. A., T. Wang, J. R. Layne, A. L. DeVries and R. E. Lee. 1995. Antifreeze glycoproteins promote lethal intracellular freezing of rat cardiomyocytes at high subzero temperatures. American Journal of Physiology 269 (Regulatory Integrative Comparative Physiology 38): R474-479. | PDF
Costanzo, J. P., J. B. Iverson, M. F. Wright and R. E. Lee. 1995. Cold hardiness and overwintering strategies of hatchlings in an assemblage of northern turtles. Ecology 76: 1772-1785. | PDF
Costanzo, J. P., C. Grenot and R. E. Lee. 1995. Supercooling, ice inoculation, and freeze tolerance in the European common lizard, Lacerta vivipara. Journal of Comparative Physiology 165: 238-244. | PDF
Layne, J. R. and R. E. Lee. 1995. Adaptations of frogs to survive
freezing. Climate
Research 5: 53-59. |
PDF
Kling,
K. B., J. P. Costanzo and R. E. Lee. 1994. Post-freeze
recovery of peripheral nerve function in the freeze-tolerant wood
frog, Rana sylvatica. Journal of Comparative Physiology
164: 316-320. | PDF
Costanzo, J. P. and R. E. Lee. 1994. Biophysical and physiological
responses promoting freeze tolerance in vertebrates. News in
Physiological Sciences 9: 252-256. | PDF
Costanzo, J. P. and R. E. Lee. 1993. Cryoprotectant production capacity
in the freeze-tolerant wood frog, Rana sylvatica. Canadian
Journal of Zoology 71: 71-75. | PDF
Costanzo, J. P., R. E. Lee and M. F. Wright. 1993. Physiological
responses to freezing in the freeze-tolerant turtle, Terrapene
carolina. Journal of Herpetology 27: 117-120. | PDF
Costanzo, J. P., R. E. Lee and P. H. Lortz. 1993. Glucose concentration
regulates freeze tolerance in the wood frog Rana sylvatica.
Journal of Experimental Biology 181: 145-155. | PDF
Costanzo, J. P., R. E. Lee and P. H. Lortz. 1993. Physiological
responses of freeze-tolerant and intolerant frogs: Clues to evolution
of anuran freeze tolerance. American Journal of Physiology
265 (Regulatory Integrative Comparative Physiology 34):
R721-725. | PDF
Costanzo, J. P., M. F. Wright and R. E. Lee. 1992. Freeze tolerance
as an overwintering adaptation in Cope's gray tree frog (Hyla
chrysoscelis). Copeia 1992: 565-569. | PDF
Costanzo, J. P., R. E. Lee, and M. F. Wright. 1992. Cooling rate
influences cryoprotectant distribution and organ dehydration in
freezing wood frogs. Journal of Experimental Zoology 26:
373-378. | PDF
Lee, R. E., J. P. Costanzo, E. C. Davidson and
J. R. Layne. 1992. Dynamics of body water during freezing and thawing
in a freeze-tolerant frog (Rana sylvatica). Journal
of Thermal Biology 17: 263-266. | PDF
Layne, J. R. and R. E. Lee. 1991. The importance of body tissue
composition to calorimetric assessments of ice content in whole
animals. Journal of Thermal Biology 16: 61-64. | PDF
Costanzo, J. P., R. E. Lee and M. F. Wright. 1991. Effect of cooling
rate on the survival of frozen wood frogs, Rana sylvatica.
Journal of Comparative Physiology B. 161: 225-229. | PDF
Costanzo, J. P., R. E. Lee and M. F. Wright. 1991. Glucose loading
prevents freezing injury in rapidly cooled frogs. American Journal
of Physiology 261 (Regulatory Integrative Comparative Physiology
30): R1549-1553. | PDF
Costanzo, J. P. and R. E. Lee. 1991. Freeze-thaw injury in erythrocytes
of the freeze-tolerant wood frog, Rana sylvatica. American
Journal of Physiology 261 (Regulatory Integrative Comparative
Physiology 30): R1346-1350. | PDF
Costanzo, J. P. and D. L. Claussen. 1990. Natural freeze tolerance
in the terrestrial turtle, Terrapene carolina. Journal
of Experimental Zoology 254: 228-232. | PDF
Layne, J. R., R. E. Lee and J. L. Huang. 1990.
Inoculation triggers freezing at high subzero temperatures in a
freeze-tolerant frog (Rana sylvatica) and insect (Eurosta
solidaginis). Canadian Journal
of Zoology 68: 506-510. | PDF
Costanzo,
J. P. 1989. Conspecific scent trailing by garter snakes during autumn:
further evidence for the use of pheromones in den location. Journal
of Chemical Ecology 15: 2531-2538. | PDF
Costanzo, J. P. 1989. Effects of humidity, temperature, and submergence
behavior on survivorship and energy use in hibernating garter snakes,
Thamnophis sirtalis. Canadian Journal of Zoology 67:
2486-2492. | PDF
Costanzo, J. P. 1989. A physiological basis for prolonged submergence
in hibernating garter snakes Thamnophis sirtalis: evidence
for an energy-sparing adaptation. Physiological Zoology
62: 580-592. | PDF
Layne, R. E. and R. E. Lee. 1989. Seasonal variation in freeze tolerance
and ice content of the tree frog Hyla versicolor. Journal
of Experimental Zoology 249: 133-137. | PDF
Layne, J. R., R. E. Lee and T. L. Heil. 1989. Freezing-induced
changes in the heart rate of wood frogs (Rana sylvatica).
American Journal of Physiology 257 (Regulatory Integrative
Comparative Physiology 26): R1046-R1049. | PDF
Costanzo, J. P., Claussen, D. L. and R. E. Lee. 1988. Natural freeze
tolerance in a reptile. Cryo-Letters 9: 380-385. | PDF
Layne, J. R. and R. E. Lee. 1987. Freeze tolerance and the dynamics
of ice formation in wood frogs (Rana sylvatica) from southern
Ohio. Canadian Journal of Zoology 65: 2062-2065. | PDF
Costanzo, J. P. 1986. Influences of hibernaculum microenvironment
on the winter life history of the garter snake, Thamnophis sirtalis.
Ohio Journal of Science 86: 199-204. | PDF
Costanzo, J. P. 1985. The bioenergetics of hibernation in the eastern
garter snake Thamnophis sirtalis sirtalis. Physiological
Zoology 58: 682-692. | PDF
Costanzo, J. P. 1982. Heating
and cooling rates of Terrapene ornata and Chrysemys
picta in water. Bios 53: 159-166. | PDF